Assay Kit For Genotyping Human Papillomavirus (PCR-Reverse Dot Blot)
It can accurately classify the 18 HPV subtypes related to cervical cancer recommended by the CFDA for testing, namely: 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59 , 66, 68, 73, 82; and can accurately classify other 10 low-risk HPV subtypes: 6, 11, 40, 42, 43, 44, 54, 61, 81, 83.
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Product Description
1. One tube reaction was used to detect 28 HPV subtypes, Can be specific classification;
2. UDG enzyme system was used to prevent PCR contamination effectively.
3. With internal and external quality control, exclude false negative, false positive reaction.
4. The sensitivity and specificity of the reagent were 98.43% and 98.91% respectively.
5. Accurate typing and different management strategies for different types of infection can help improve sensitivity and control colposcopy referral rates.
6. Predict the risk degree of patients and determine the screening interval.
7. Typing tests distinguish persistent infection from transient infection.
8. Distinguish single infection from multiple infection.
Product Features
1. Advanced and unique technology
One tube reaction detects multiple HPV subtypes;
Set up internal reference system to eliminate false negative results;
The use of hot-start enzymes improves the specificity of PCR amplification;
The multiple PCR buffer with super buffering capacity is used to ensure the sensitivity of detection.
2. Good product applicability, convenient and quick detection
Qualitative PCR system, suitable for a variety of qualitative PCR machines, the hybridization process can use automatic nucleic acid molecular hybridization instrument (LBP-3124) or manual rapid hybridization;
The detection is not limited by the number of samples, and the whole process only takes 4-5 hours.
3. Guaranteed test results
Adopt UDG enzyme system to effectively prevent PCR contamination;
With internal and external quality control, false negative and false positive reactions are excluded;
The results of clinical trials showed that the sensitivity of the reagent was 98.43% and the specificity was 98.91%.
4. Comply with the latest international cervical cancer screening strategy
The classification of HPV16/18 can help improve sensitivity and control the referral rate of colposcopy;
Predict the subject's risk of disease and determine the screening interval;
Type detection to distinguish between persistent infection and transient infection;
Distinguish between single infection and multiple infections;
Accurate classification, adopt different treatment plans for different types of infections.
PCR-RDB Process
Sample extraction (30min) → Sample loading (10-15mini) → PCR (1.5-2h) → Hybridization (1.5-2h) → Result Reading (10min)
Unique Integrated Solution
With the company's liquid-based cytology products, one sampling and two detections can be realized, forming an integrated comprehensive detection program.
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